IL-21 ELISA Kit
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- Target See all IL-21 (IL21) ELISA Kits
- IL-21 (IL21) (Interleukin 21 (IL21))
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Reactivity
- Dog
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Canine (dog) IL-21 ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair recognizes Canine (dog) IL-21.
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Interleukin 21 (IL21) ELISA Kit
IL21 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 21 (IL21) ELISA KitIL21 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 21 (IL21) ELISA KitIL21 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 21 (IL21) ELISA KitSmall Sample IL21 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 21 (IL21) ELISA KitIL21 Reactivity: Mouse AA 25-146 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
Interleukin 21 (IL21) ELISA KitIL21 Reactivity: Human Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 21 (IL21) ELISA KitIL21 Reactivity: Cow Colorimetric Sandwich ELISA 0.41-100 ng/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 21 (IL21) ELISA KitIL21 Reactivity: Mouse Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
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- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 6 months
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- Target See all IL-21 (IL21) ELISA Kits
- IL-21 (IL21) (Interleukin 21 (IL21))
- Alternative Name
- IL-21 (IL21 Products)
- Synonyms
- IL-21 ELISA Kit, Za11 ELISA Kit, interleukin 21 ELISA Kit, IL21 ELISA Kit, Il21 ELISA Kit
- Gene ID
- 442935
- UniProt
- Q6L7I9
- Pathways
- JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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