NPW ELISA Kit
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- Target See all NPW ELISA Kits
- NPW (Neuropeptide W (NPW))
- Reactivity
- Human, Mouse, Rat
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Detection Range
- 0.1 ng/mL - 1000 ng/mL
- Minimum Detection Limit
- 0.1 ng/mL
- Application
- ELISA
- Purpose
- Human/Mouse/Rat NPW23 EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.
- Sample Type
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificity
- This EIA kit is designed to detect human, mouse, and rat Dynorphin
- Sensitivity
- 0.3 ng/mL
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
- Reagent Preparation
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Recommended Dilution for serum and plasma samplesHuman: sample extraction / Mouse: sample extraction / Rat: sample extraction
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
- Expiry Date
- 6 months
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- Target See all NPW ELISA Kits
- NPW (Neuropeptide W (NPW))
- Alternative Name
- NPW23 (NPW Products)
- Synonyms
- L8 ELISA Kit, L8C ELISA Kit, PPL8 ELISA Kit, PPNPW ELISA Kit, Gm935 ELISA Kit, PREPRO-NPW ELISA Kit, neuropeptide W ELISA Kit, NPW ELISA Kit, Npw ELISA Kit
- Gene ID
- 283869
- UniProt
- Q8N729
- Pathways
- Feeding Behaviour
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