CDK1 ELISA Kit

Catalog No. ABIN5690739

Product Details CDK1 ELISA Kit

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Rat, Mouse

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Purpose: Human, Mouse, and Rat Phospho-CDK1 (Y15) Cell-Based ELISA Kit. This assay semi-quantitatively measures CDK1 phosphorylated at Tyrosine-15 in adherent cell lines.

Sample Type: Cell Culture Cells

Analytical Method: Semi-Quantitative

Specificity: The antibodies provided in this kit recognizes Human CDK1 phosphorylated at site Tyrosine-15.

Characteristics:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Components:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Plate: Uncoated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDK1 (CDK1 Products)

Synonyms: CDC2 ELISA Kit, CDC28A ELISA Kit, P34CDC2 ELISA Kit, Cdc2 ELISA Kit, Cdc2a ELISA Kit, p34 ELISA Kit, cdc2 ELISA Kit, wu:fc30e01 ELISA Kit, zgc:92032 ELISA Kit, PSTAIR ELISA Kit, cdc-2 ELISA Kit, cdc2-a ELISA Kit, cdc28a ELISA Kit, cdc2x1.1 ELISA Kit, p34cdc2 ELISA Kit, xcdc2 ELISA Kit, 5363 ELISA Kit, CDCDm ELISA Kit, CDK1 ELISA Kit, CDK1/CDC2 ELISA Kit, CG5363 ELISA Kit, Cdk-1 ELISA Kit, Cdk1 ELISA Kit, Dcdc2 ELISA Kit, Dm cdc2 ELISA Kit, DmCdc2 ELISA Kit, DmCdk1 ELISA Kit, Dmcdc2 ELISA Kit, Dmel\\CG5363 ELISA Kit, cdc ELISA Kit, cdc2Dm ELISA Kit, cdk1 ELISA Kit, dCdk1 ELISA Kit, group 4 ELISA Kit, l(2)31Eh ELISA Kit, CDC2A ELISA Kit, CDC2AAT ELISA Kit, CDK2 ELISA Kit, CDKA1 ELISA Kit, CDKA;1 ELISA Kit, CYCLIN-DEPENDENT KINASE A;1 ELISA Kit, cell division control 2 ELISA Kit, cdc2-b ELISA Kit, cdc2a ELISA Kit, cdc2x1.2 ELISA Kit, POL3 ELISA Kit, pold ELISA Kit, cyclin dependent kinase 1 ELISA Kit, cyclin dependent kinase like 1 ELISA Kit, Cell division control protein 2 1 ELISA Kit, cyclin-dependent kinase 1 ELISA Kit, cyclin-dependent kinase 1 S homeolog ELISA Kit, Cyclin-dependent kinase 1 ELISA Kit, cell division control 2 ELISA Kit, cell division control protein2 homolog ELISA Kit, cyclin-dependent kinase 1 L homeolog ELISA Kit, polymerase (DNA directed), delta 1, catalytic subunit L homeolog ELISA Kit, cyclin-dependent protein kinase Cdk1/Cdc2 ELISA Kit, CDK1 ELISA Kit, CDKL1 ELISA Kit, POPTR_0004s14080g ELISA Kit, Cdk1 ELISA Kit, cdk1 ELISA Kit, cdk1.S ELISA Kit, CDC2 ELISA Kit, cdc2 ELISA Kit, cdk-1 ELISA Kit, cdk1.L ELISA Kit, pold1.L ELISA Kit

Gene ID: 983

UniProt: P06493, P11440, P39951

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA