RPS6KA3 ELISA Kit

Catalog No. ABIN5690735

Product Details RPS6KA3 ELISA Kit

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Purpose: Human, Mouse and Rat Phospho-RSK2 (Ser386) and Total RSK2 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures RSK2 phosphorylated at Serine-386 as well as total RSK2 in adherent cell lines.

Sample Type: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificity: The antibodies provided in this kit recognizes Human, Mouse and Rat RSK2 phosphorylated at site Serine-386 as well as total RSK2.

Characteristics:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Components:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Plate: Uncoated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for RPS6KA3

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Alternative Name: RSK2 (RPS6KA3 Products)

Synonyms: HU-1 ELISA Kit, MAPKAPK1A ELISA Kit, RSK ELISA Kit, RSK1 ELISA Kit, CLS ELISA Kit, HU-3 ELISA Kit, ISPK-1 ELISA Kit, MAPKAPK1B ELISA Kit, MRX19 ELISA Kit, RSK2 ELISA Kit, S6K-alpha3 ELISA Kit, p90-RSK2 ELISA Kit, pp90RSK2 ELISA Kit, MPK-9 ELISA Kit, Rsk2 ELISA Kit, RGD1563860 ELISA Kit, id:ibd1103 ELISA Kit, rps6ka3 ELISA Kit, si:ch211-114c12.12 ELISA Kit, wu:fk86e02 ELISA Kit, zgc:56006 ELISA Kit, zgc:158386 ELISA Kit, ribosomal protein S6 kinase A1 ELISA Kit, ribosomal protein S6 kinase A3 ELISA Kit, ribosomal protein S6 kinase polypeptide 3 ELISA Kit, ribosomal protein S6 kinase a, polypeptide 3a ELISA Kit, ribosomal protein S6 kinase, polypeptide 3b ELISA Kit, RPS6KA1 ELISA Kit, RPS6KA3 ELISA Kit, Rps6ka3 ELISA Kit, rps6ka3a ELISA Kit, rps6ka3b ELISA Kit

Gene ID: 6197

UniProt: P51812, P18654, D3Z8E0

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades