ALDH7A1 ELISA Kit

Catalog No. ABIN5674954

Product Details ALDH7A1 ELISA Kit

Target: ALDH7A1 (Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0133 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ALDH7A1

Target: ALDH7A1 (Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1))

Abstract: ALDH7A1 Products

Synonyms: atq1 ELISA Kit, ALDH7A1 ELISA Kit, ATQ1 ELISA Kit, EPD ELISA Kit, PDE ELISA Kit, antiquitin ELISA Kit, ATQ ELISA Kit, wu:fi34d12 ELISA Kit, wu:fi35e06 ELISA Kit, Atq1 ELISA Kit, D18Wsu181e ELISA Kit, aldehyde dehydrogenase 7 family member A1 ELISA Kit, aldehyde dehydrogenase 7 family member A1 L homeolog ELISA Kit, aldehyde dehydrogenase 7 family, member A1 ELISA Kit, aldehyde dehydrogenase family 7, member A1 ELISA Kit, ALDH7A1 ELISA Kit, aldh7a1 ELISA Kit, aldh7a1.L ELISA Kit, Aldh7a1 ELISA Kit

Background: Alternative name: ATQ1, PDE, EPD, Antiquitin, Alpha-Aminoadipic Semialdehyde Dehydrogenase, Delta1-Piperideine-6-Carboxylate Dehydrogenease, P6c Dehydrogenase

Gene ID: 291450

UniProt: Q64057

Pathways: Sensory Perception of Sound