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JKAMP ELISA Kit

JKAMP Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
Catalog No. ABIN5674944
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    JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))
    Reactivity
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.781 ng/mL - 50 ng/mL
    Minimum Detection Limit
    0.781 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0.35 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
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    JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))
    Alternative Name
    JNK1/MAPK8 Associated Membrane Protein (JKAMP) (JKAMP Products)
    Synonyms
    C14orf100 ELISA Kit, HSPC213 ELISA Kit, HSPC327 ELISA Kit, JAMP ELISA Kit, 1200003C05Rik ELISA Kit, 2310047L11Rik ELISA Kit, Jamp ELISA Kit, RGD1308917 ELISA Kit, JNK1/MAPK8 associated membrane protein ELISA Kit, JNK1/MAPK8-associated membrane protein ELISA Kit, JKAMP ELISA Kit, Jkamp ELISA Kit
    Background
    Alternative name: JAMP, C14orf100, Jun N-Terminal Kinase 1-Associated Membrane Protein, Medulloblastoma antigen MU-MB-50.4
    Gene ID
    51528
    UniProt
    Q9P055
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