TREH ELISA Kit

Catalog No. ABIN5674939

Product Details TREH ELISA Kit

Target: TREH (Trehalase (Brush-Border Membrane Glycoprotein) (TREH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0224 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for TREH

Target: TREH (Trehalase (Brush-Border Membrane Glycoprotein) (TREH))

Alternative Name: Trehalase (TREH) (TREH Products)

Synonyms: 87D3T ELISA Kit, CG9364 ELISA Kit, Dmel\\CG9364 ELISA Kit, ESTS:87D3T ELISA Kit, TRE ELISA Kit, TREH ELISA Kit, Tre ELISA Kit, anon-EST:Posey159 ELISA Kit, anon-sts38 ELISA Kit, GB16316 ELISA Kit, TREA ELISA Kit, 2210412M19Rik ELISA Kit, Trehalase ELISA Kit, trehalase ELISA Kit, trehalase (brush-border membrane glycoprotein) ELISA Kit, Treh ELISA Kit, LOC693027 ELISA Kit, LOC410795 ELISA Kit, TREH ELISA Kit

Background: Alternative name: TRE, TREA, Brush Border Membrane Glycoprotein, Alpha,Alpha-Trehalase, Alpha,Alpha-Trehalose Glucohydrolase

Gene ID: 11181

UniProt: O43280