LKB1 ELISA Kit

Catalog No. ABIN5674931

Product Details LKB1 ELISA Kit

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0057 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for LKB1

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Abstract: STK11 Products

Synonyms: pjs ELISA Kit, LKB1 ELISA Kit, XEEK1 ELISA Kit, Stk11 ELISA Kit, PJS ELISA Kit, hLKB1 ELISA Kit, AA408040 ELISA Kit, Lkb1 ELISA Kit, Par-4 ELISA Kit, R75140 ELISA Kit, mLKB1 ELISA Kit, wu:fj61a05 ELISA Kit, zgc:110180 ELISA Kit, xeek1 ELISA Kit, serine/threonine kinase 11 ELISA Kit, polarization-related protein LKB1 ELISA Kit, serine/threonine kinase 11 L homeolog ELISA Kit, STK11 ELISA Kit, stk11 ELISA Kit, LOC662493 ELISA Kit, Stk11 ELISA Kit, stk11.L ELISA Kit

Background: Alternative name: LKB1, PJS, Polarization-Related Protein LKB1, Peutz-Jeghers Syndrome, Liver kinase B1, Renal carcinoma antigen NY-REN-19

UniProt: D4AE59

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect