PDE3B ELISA Kit
PDE3B
Reactivity: Human
Colorimetric
Sandwich ELISA
78.125 pg/mL - 5000 pg/mL
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- Target See all PDE3B products
- PDE3B (phosphodiesterase 3B, CGMP-Inhibited (PDE3B))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 78.125 pg/mL - 5000 pg/mL
- Minimum Detection Limit
- 78.125 pg/mL
- Application
- ELISA
- Sample Type
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 26.35 pg/mL
- Components
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Material not included
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target See all PDE3B products
- PDE3B (phosphodiesterase 3B, CGMP-Inhibited (PDE3B))
- Alternative Name
- Phosphodiesterase 3B, cGMP Inhibited (PDE3B) (PDE3B Products)
- Synonyms
- PDE3B ELISA Kit, HcGIP1 ELISA Kit, cGIPDE1 ELISA Kit, 9830102A01Rik ELISA Kit, AI847709 ELISA Kit, phosphodiesterase 3B ELISA Kit, phosphodiesterase 3B, cGMP-inhibited ELISA Kit, si:ch211-79e4.4 ELISA Kit, phosphodiesterase 3B L homeolog ELISA Kit, PDE3B ELISA Kit, pde3b ELISA Kit, Pde3b ELISA Kit, si:ch211-79e4.4 ELISA Kit, pde3b.L ELISA Kit
- Background
- Alternative name: HcGIP1, cGIPDE1, CGIPDE1, CGIP1, CGI-PDE B, Cyclic GMP-inhibited phosphodiesterase B
- Gene ID
- 5140
- UniProt
- Q13370
- Pathways
- Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, cAMP Metabolic Process
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