NME1 ELISA Kit

Catalog No. ABIN5674914

Product Details NME1 ELISA Kit

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Sensitivity: 0053 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for NME1

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Alternative Name: Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) (NME1 Products)

Synonyms: AWD ELISA Kit, GAAD ELISA Kit, NB ELISA Kit, NBS ELISA Kit, NDKA ELISA Kit, NDPK-A ELISA Kit, NDPKA ELISA Kit, NM23 ELISA Kit, NM23-H1 ELISA Kit, AL024257 ELISA Kit, NM23-M1 ELISA Kit, NM23A ELISA Kit, NDPK-Z1 ELISA Kit, NM23-B ELISA Kit, NM23-Z1 ELISA Kit, ndpkz1 ELISA Kit, nm23b ELISA Kit, nme1 ELISA Kit, nme2 ELISA Kit, nme2b1 ELISA Kit, NME1 ELISA Kit, NME1-NME2 ELISA Kit, NM23-C1 ELISA Kit, ndpk-b ELISA Kit, ndpkb ELISA Kit, nm23-h2 ELISA Kit, nm23ndk ELISA Kit, puf ELISA Kit, NBR-A ELISA Kit, NBR-B ELISA Kit, NME1-1 ELISA Kit, nm23ndk-a ELISA Kit, NDK I ELISA Kit, NDPK I ELISA Kit, T30A10.80 ELISA Kit, T30A10_80 ELISA Kit, NDP kinase I ELISA Kit, NME/NM23 nucleoside diphosphate kinase 1 ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 ELISA Kit, non-metastatic cells 1, protein (NM23A) expressed in ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2 S homeolog ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2 L homeolog ELISA Kit, nucleoside diphosphate kinase ELISA Kit, nucleoside diphosphate kinase 1 ELISA Kit, NME1 ELISA Kit, Nme1 ELISA Kit, nme2b.1 ELISA Kit, nme2.S ELISA Kit, nme2.L ELISA Kit, LOC547870 ELISA Kit, NDPK1 ELISA Kit, LOC107790563 ELISA Kit

Background: Alternative name: AWD, GAAD, NDPKA, NM23-H1, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein

Gene ID: 18102

UniProt: P15532

Pathways: Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process