BAT3 ELISA Kit

Catalog No. ABIN5674908

Product Details BAT3 ELISA Kit

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.125 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 36 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for BAT3

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Alternative Name: HLA-B Associated Transcript 3 (BAT3) (BAT3 Products)

Synonyms: ARABIDOPSIS THALIANA BCL-2-ASSOCIATED ATHANOGENE 6 ELISA Kit, ATBAG6 ELISA Kit, BCL-2-associated athanogene 6 ELISA Kit, T3F17.11 ELISA Kit, 2410045D21Rik ELISA Kit, AA408914 ELISA Kit, BAG-6 ELISA Kit, Bat3 ELISA Kit, D17H6S52E ELISA Kit, G3 ELISA Kit, Scythe ELISA Kit, BAT3 ELISA Kit, D6S52E ELISA Kit, BCL-2-associated athanogene 6 ELISA Kit, BCL2-associated athanogene 6 ELISA Kit, BCL2 associated athanogene 6 ELISA Kit, BAG6 ELISA Kit, Bag6 ELISA Kit

Background: Alternative name: BAG6, Protein G3, Protein Scythe, BCL2-associated athanogene 6, BAG family molecular chaperone regulator 6, Large proline-rich protein BAG6

Gene ID: 7917

UniProt: P46379