RGS6 ELISA Kit
RGS6
Reactivity: Mouse
Colorimetric
Sandwich ELISA
0.156 ng/mL - 10 ng/mL
Tissue Homogenate
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- Target See all RGS6 ELISA Kits
- RGS6 (Regulator of G-Protein Signaling 6 (RGS6))
-
Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 0066 ng/mL
- Components
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Material not included
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product RGS6 ELISA Kit
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Regulator of G-Protein Signaling 6 (RGS6) ELISA Kit
RGS6 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target See all RGS6 ELISA Kits
- RGS6 (Regulator of G-Protein Signaling 6 (RGS6))
- Alternative Name
- Regulator Of G Protein Signaling 6 (RGS6) (RGS6 Products)
- Synonyms
- RGS6 ELISA Kit, DKFZp459O0734 ELISA Kit, GAP ELISA Kit, hm:zeh0300 ELISA Kit, si:ch211-268e23.2 ELISA Kit, regulator of G protein signaling 6 ELISA Kit, regulator of G-protein signaling 6 ELISA Kit, regulator of G-protein signaling 6 S homeolog ELISA Kit, RGS6 ELISA Kit, Rgs6 ELISA Kit, rgs6.S ELISA Kit, rgs6 ELISA Kit
- Background
- Alternative name: GAP, S914
- Gene ID
- 50779
- UniProt
- Q9Z2H2
- Pathways
- Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling
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