EPHX1 ELISA Kit

Catalog No. ABIN5674888

Product Details EPHX1 ELISA Kit

Target: EPHX1 (Epoxide Hydrolase 1, Microsomal (Xenobiotic) (EPHX1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 29.3 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for EPHX1

Target: EPHX1 (Epoxide Hydrolase 1, Microsomal (Xenobiotic) (EPHX1))

Alternative Name: Epoxide Hydrolase 1, Microsomal (EPHX1) (EPHX1 Products)

Synonyms: NV13267 ELISA Kit, DRB1 ELISA Kit, DSRNA-BINDING PROTEIN 1 ELISA Kit, F21M12.9 ELISA Kit, F21M12_9 ELISA Kit, HYPONASTIC LEAVES 1 ELISA Kit, AI195553 ELISA Kit, Eph-1 ELISA Kit, Eph1 ELISA Kit, mEH ELISA Kit, EPHX ELISA Kit, EPOX ELISA Kit, HYL1 ELISA Kit, MEH ELISA Kit, zgc:56126 ELISA Kit, ephx ELISA Kit, epox ELISA Kit, meh ELISA Kit, MEH8 ELISA Kit, epoxide hydrolase 1 ELISA Kit, epoxide hydrolase 1, microsomal (xenobiotic) ELISA Kit, Epoxide hydrolase 1 ELISA Kit, dsRNA-binding domain-like superfamily protein ELISA Kit, epoxide hydrolase 1, microsomal ELISA Kit, epoxide hydrolase 1, microsomal (xenobiotic) L homeolog ELISA Kit, EPHX1 ELISA Kit, Eh1 ELISA Kit, hyep ELISA Kit, HYL1 ELISA Kit, Ephx1 ELISA Kit, ephx1 ELISA Kit, ephx1.L ELISA Kit

Background: Alternative name: Epoxide hydratase, Microsomal epoxide hydrolase, Eph-1, MEH8

Gene ID: 13849

UniProt: Q9D379