PYCARD ELISA Kit

Catalog No. ABIN5674867

Product Details PYCARD ELISA Kit

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0065 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: PYD And CARD Domain Containing Protein (PYCARD) (PYCARD Products)

Synonyms: ASC ELISA Kit, CARD5 ELISA Kit, TMS ELISA Kit, TMS-1 ELISA Kit, TMS1 ELISA Kit, 9130417A21Rik ELISA Kit, Asc ELISA Kit, TNS1 ELISA Kit, masc ELISA Kit, asc1 ELISA Kit, zAsc ELISA Kit, zgc:109874 ELISA Kit, PYD and CARD domain containing ELISA Kit, PYCARD ELISA Kit, Pycard ELISA Kit, pycard ELISA Kit

Background: Alternative name: ASC, CARD5, TMS1, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, Target of methylation-induced silencing 1

Gene ID: 29108

UniProt: Q9ULZ3

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome