ACAT1 ELISA Kit

Catalog No. ABIN5674861

Product Details ACAT1 ELISA Kit

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 12.5 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Alternative Name: Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) (ACAT1 Products)

Synonyms: ACAT ELISA Kit, MAT ELISA Kit, T2 ELISA Kit, THIL ELISA Kit, RATACAL ELISA Kit, 6330585C21Rik ELISA Kit, Acat ELISA Kit, fd16h07 ELISA Kit, fd20g06 ELISA Kit, wu:fd16h07 ELISA Kit, wu:fd20g06 ELISA Kit, zgc:86832 ELISA Kit, acat1-a ELISA Kit, acetyl-CoA acetyltransferase 1 ELISA Kit, acetyl-Coenzyme A acetyltransferase 1 ELISA Kit, acetyl-CoA acetyltransferase 1 L homeolog ELISA Kit, ACAT1 ELISA Kit, Acat1 ELISA Kit, acat1 ELISA Kit, acat1.L ELISA Kit

Background: Alternative name: THIL, ACAT, T2, MAT, Acetoacetyl Coenzyme A Thiolase

Gene ID: 25014

UniProt: P17764