PAOX ELISA Kit
PAOX
Reactivity: Human
Colorimetric
Sandwich ELISA
78.125 pg/mL - 5000 pg/mL
Plasma, Serum, Tissue Homogenate
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- Target See all PAOX ELISA Kits
- PAOX (Polyamine Oxidase (Exo-N4-Amino) (PAOX))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 78.125 pg/mL - 5000 pg/mL
- Minimum Detection Limit
- 78.125 pg/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 31.5 pg/mL
- Components
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Material not included
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product PAOX ELISA Kit
-
Polyamine Oxidase (Exo-N4-Amino) (PAOX) ELISA Kit
PAOX Reactivity: Mouse Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target See all PAOX ELISA Kits
- PAOX (Polyamine Oxidase (Exo-N4-Amino) (PAOX))
- Alternative Name
- Polyamine Oxidase (PAOX) (PAOX Products)
- Synonyms
- PAO ELISA Kit, mpao1 ELISA Kit, 2410012F02Rik ELISA Kit, AI118225 ELISA Kit, Pao ELISA Kit, pao ELISA Kit, polyamine oxidase ELISA Kit, polyamine oxidase 1 ELISA Kit, amine oxidase family protein ELISA Kit, si:dkey-275b16.2 ELISA Kit, polyamine oxidase (exo-N4-amino) ELISA Kit, polyamine oxidase (exo-N4-amino) L homeolog ELISA Kit, PAOX ELISA Kit, pao1 ELISA Kit, NFIA_077590 ELISA Kit, POPTR_0011s12590g ELISA Kit, si:dkey-275b16.2 ELISA Kit, Paox ELISA Kit, paox.L ELISA Kit
- Background
- Alternative name: PAO, Peroxisomal N(1)-Acetyl-Spermine/Spermidine Oxidase(Exo-N4-Amino)
- Gene ID
- 196743
- UniProt
- Q6QHF9
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