SGPP2 ELISA Kit
SGPP2
Reactivity: Human
Colorimetric
Sandwich ELISA
0.156 ng/mL - 10 ng/mL
Tissue Homogenate
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- Target See all SGPP2 ELISA Kits
- SGPP2 (Sphingosine-1-Phosphate Phosphatase 2 (SGPP2))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 0059 ng/mL
- Components
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Material not included
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product SGPP2 ELISA Kit
-
Sphingosine-1-Phosphate Phosphatase 2 (SGPP2) ELISA Kit
SGPP2 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target See all SGPP2 ELISA Kits
- SGPP2 (Sphingosine-1-Phosphate Phosphatase 2 (SGPP2))
- Abstract
- SGPP2 Products
- Synonyms
- SPP2 ELISA Kit, RGD1565739 ELISA Kit, SPPase2 ELISA Kit, Spp2 ELISA Kit, sphingosine-1-phosphate phosphatase 2 ELISA Kit, sphingosine-1-phosphate phosphotase 2 ELISA Kit, SGPP2 ELISA Kit, Sgpp2 ELISA Kit
- Background
- Alternative name: SPP2
- Gene ID
- 130367
- UniProt
- Q8IWX5
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