DSTYK ELISA Kit

Catalog No. ABIN5674835

Product Details DSTYK ELISA Kit

Target: DSTYK (Dual serine/threonine and tyrosine Protein Kinase (DSTYK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0055 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for DSTYK

Target: DSTYK (Dual serine/threonine and tyrosine Protein Kinase (DSTYK))

Abstract: DSTYK Products

Synonyms: CAKUT1 ELISA Kit, DustyPK ELISA Kit, RIP5 ELISA Kit, RIPK5 ELISA Kit, A930019K20Rik ELISA Kit, C430014H23Rik ELISA Kit, C820013G01 ELISA Kit, Ripk5 ELISA Kit, ripk5 ELISA Kit, zgc:136421 ELISA Kit, DSTYK ELISA Kit, GB11994 ELISA Kit, DUSTYPK ELISA Kit, dual serine/threonine and tyrosine protein kinase ELISA Kit, receptor interacting protein kinase 5 ELISA Kit, dual serine/threonine and tyrosine protein kinase S homeolog ELISA Kit, DSTYK ELISA Kit, Dstyk ELISA Kit, dstyk ELISA Kit, Ripk5 ELISA Kit, dstyk.S ELISA Kit

Background: Alternative name: RIPK5, Dusty PK, RIP5, SgK496, Receptor Interacting Protein Kinase 5, RIP-homologous kinase, Sugen kinase 496, Receptor-interacting serine/threonine-protein kinase 5

Gene ID: 25778

UniProt: Q6XUX3