ASS1 ELISA Kit

Catalog No. ABIN5674811

Product Details ASS1 ELISA Kit

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0071 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ASS1

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Alternative Name: Argininosuccinate Synthetase 1 (ASS1) (ASS1 Products)

Synonyms: ASS ELISA Kit, CTLN1 ELISA Kit, AA408052 ELISA Kit, Ass-1 ELISA Kit, fold ELISA Kit, ASSA ELISA Kit, Ass ELISA Kit, ass ELISA Kit, zgc:92051 ELISA Kit, wu:fb95a04 ELISA Kit, wu:fc01e08 ELISA Kit, argininosuccinate synthase 1 ELISA Kit, argininosuccinate synthetase 1 ELISA Kit, argininosuccinate synthase 1 S homeolog ELISA Kit, ASS1 ELISA Kit, Ass1 ELISA Kit, ass1.S ELISA Kit, ass1 ELISA Kit

Background: Alternative name: CTLN1, Citrulline--aspartate ligase

Gene ID: 25698

UniProt: P09034

Pathways: Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin