Arc ELISA Kit

Catalog No. ABIN5674809

Product Details Arc ELISA Kit

Target: Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.125 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 31 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for Arc

Target: Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))

Alternative Name: Activity Regulated Cytoskeleton Associated Protein (ARC) (Arc Products)

Synonyms: ARC ELISA Kit, arg3.1 ELISA Kit, Arc3.1 ELISA Kit, C86064 ELISA Kit, mArc ELISA Kit, Arg3.1 ELISA Kit, rg3.1 ELISA Kit, ARG3.1 ELISA Kit, activity regulated cytoskeleton associated protein ELISA Kit, activity-regulated cytoskeleton-associated protein ELISA Kit, activity regulated cytoskeletal-associated protein ELISA Kit, ARC ELISA Kit, arc ELISA Kit, Arc ELISA Kit

Background: Alternative name: Arg3.1, Activity-regulated gene 3.1 protein homolog

Gene ID: 23237

UniProt: Q7LC44