HTR2C ELISA Kit

Catalog No. ABIN5674800

Product Details HTR2C ELISA Kit

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.125 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 26.6 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for HTR2C

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Alternative Name: 5-Hydroxytryptamine Receptor 2C (HTR2C) (HTR2C Products)

Synonyms: 5-HT2C ELISA Kit, 5-HTR2C ELISA Kit, HTR1C ELISA Kit, 5-HT2cR ELISA Kit, 5HT1c ELISA Kit, Htr1c ELISA Kit, SR1 ELISA Kit, 5HT-1C ELISA Kit, 5HT2C ELISA Kit, 5-HT-2C ELISA Kit, 5-ht2cr ELISA Kit, si:dkey-24g18.2 ELISA Kit, 5ht2c ELISA Kit, 5HTR2C ELISA Kit, 5-hydroxytryptamine receptor 2C ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled S homeolog ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled-like 1 ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled ELISA Kit, HTR2C ELISA Kit, Htr2c ELISA Kit, htr2c.S ELISA Kit, htr2cl1 ELISA Kit, LOC407134 ELISA Kit

Background: Alternative name: 5-HT2C, HTR1C, Serotonin Receptor 2C, 5-hydroxytryptamine receptor 1C

Gene ID: 15560

UniProt: P34968

Pathways: Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour