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HTR2C ELISA Kit

HTR2C Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Catalog No. ABIN5674799
  • Target See all HTR2C ELISA Kits
    HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))
    Reactivity
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0064 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product HTR2C ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all HTR2C ELISA Kits
    HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))
    Alternative Name
    5-Hydroxytryptamine Receptor 2C (HTR2C) (HTR2C Products)
    Synonyms
    5-HT2C ELISA Kit, 5-HTR2C ELISA Kit, HTR1C ELISA Kit, 5-HT2cR ELISA Kit, 5HT1c ELISA Kit, Htr1c ELISA Kit, SR1 ELISA Kit, 5HT-1C ELISA Kit, 5HT2C ELISA Kit, 5-HT-2C ELISA Kit, 5-ht2cr ELISA Kit, si:dkey-24g18.2 ELISA Kit, 5ht2c ELISA Kit, 5HTR2C ELISA Kit, 5-hydroxytryptamine receptor 2C ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled S homeolog ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled-like 1 ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled ELISA Kit, HTR2C ELISA Kit, Htr2c ELISA Kit, htr2c.S ELISA Kit, htr2cl1 ELISA Kit, LOC407134 ELISA Kit
    Background
    Alternative name: 5-HT2C, HTR1C, Serotonin Receptor 2C, 5-hydroxytryptamine receptor 1C
    Gene ID
    3358
    UniProt
    P28335
    Pathways
    Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour
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