NRN1 ELISA Kit

Catalog No. ABIN5674796

Product Details NRN1 ELISA Kit

Target: NRN1 (Neuritin 1 (NRN1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 11.5 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for NRN1

Target: NRN1 (Neuritin 1 (NRN1))

Abstract: NRN1 Products

Synonyms: NRN ELISA Kit, dJ380B8.2 ELISA Kit, 0710008J23Rik ELISA Kit, Cpg15 ELISA Kit, Nrn ELISA Kit, neuritin ELISA Kit, nrn1 ELISA Kit, zgc:92828 ELISA Kit, NRN1 ELISA Kit, cpg15 ELISA Kit, nrn ELISA Kit, nrn1-a ELISA Kit, nrn1-b ELISA Kit, nrn1b ELISA Kit, neuritin 1 ELISA Kit, neuritin 1a ELISA Kit, neuritin 1 S homeolog ELISA Kit, neuritin 1 L homeolog ELISA Kit, NRN1 ELISA Kit, Nrn1 ELISA Kit, nrn1a ELISA Kit, nrn1 ELISA Kit, nrn1.S ELISA Kit, nrn1.L ELISA Kit

Gene ID: 51299

UniProt: Q9NPD7