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NEU2 ELISA Kit

NEU2 Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5674767
  • Target See all NEU2 ELISA Kits
    NEU2 (Sialidase 2 (Cytosolic Sialidase) (NEU2))
    Reactivity
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0082 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product NEU2 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all NEU2 ELISA Kits
    NEU2 (Sialidase 2 (Cytosolic Sialidase) (NEU2))
    Alternative Name
    Sialidase 2, Cytosolic (SIAL2) (NEU2 Products)
    Synonyms
    NEU2 ELISA Kit, MBS ELISA Kit, MSS ELISA Kit, MTS ELISA Kit, SIAL2 ELISA Kit, neuraminidase 2 ELISA Kit, sialidase 2 (cytosolic sialidase) ELISA Kit, NEU2 ELISA Kit, Neu2 ELISA Kit
    Background
    Alternative name: NEU2, N-Acetyl-Alpha-Neuraminidase 2, Cytosolic sialidase
    Gene ID
    4759
    UniProt
    Q9Y3R4
    Pathways
    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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