KCNJ5 ELISA Kit

Catalog No. ABIN5674766

Product Details KCNJ5 ELISA Kit

Target: KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0059 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for KCNJ5

Target: KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))

Alternative Name: Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) (KCNJ5 Products)

Synonyms: CIR ELISA Kit, GIRK4 ELISA Kit, KATP1 ELISA Kit, KIR3.4 ELISA Kit, LQT13 ELISA Kit, Kir3.4 ELISA Kit, GIRK-4 ELISA Kit, KATP-1 ELISA Kit, potassium voltage-gated channel subfamily J member 5 ELISA Kit, potassium inwardly-rectifying channel, subfamily J, member 5 ELISA Kit, KCNJ5 ELISA Kit, Kcnj5 ELISA Kit

Background: Alternative name: Kir3.4, CIR, KATP1, GIRK4, G protein-activated inward rectifier potassium channel 4, Cardiac inward rectifier, Inward rectifier K(+) channel Kir3.4, Heart KATP channel

Gene ID: 16521

UniProt: P48545

Pathways: Notch Signaling