TPM2 ELISA Kit

Catalog No. ABIN5674763

Product Details TPM2 ELISA Kit

Target: TPM2 (Tropomyosin-2 (TPM2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0278 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for TPM2

Target: TPM2 (Tropomyosin-2 (TPM2))

Alternative Name: Tropomyosin 2 Beta (TPM2) (TPM2 Products)

Synonyms: CG4843 ELISA Kit, DROTROPI1 ELISA Kit, Dmel\\CG4843 ELISA Kit, Ifm(3)3 ELISA Kit, Ifm(3)5 ELISA Kit, Ifm-TmI ELISA Kit, TM2 ELISA Kit, Tm ELISA Kit, Tm1 ELISA Kit, Tm127 ELISA Kit, TmI ELISA Kit, TmII ELISA Kit, Tn-H ELISA Kit, dro Tm ELISA Kit, l(3)nc99Eb ELISA Kit, mTmI ELISA Kit, cb836 ELISA Kit, fb68h02 ELISA Kit, tpm4l ELISA Kit, wu:fb68h02 ELISA Kit, wu:fj63f03 ELISA Kit, zgc:86810 ELISA Kit, AMCD1 ELISA Kit, DA1 ELISA Kit, DA2B ELISA Kit, TMSB ELISA Kit, nem4 ELISA Kit, tpm2 ELISA Kit, tpm2a ELISA Kit, BRT-2 ELISA Kit, TPM3 ELISA Kit, NEM4 ELISA Kit, Tpm-2 ELISA Kit, Trop-2 ELISA Kit, Tropomyosin 2 ELISA Kit, Tropomyosin-2 ELISA Kit, tropomyosin ELISA Kit, tropomyosin 2 (beta) ELISA Kit, tropomyosin 2 L homeolog ELISA Kit, tropomyosin 2 ELISA Kit, tropomyosin 2, beta ELISA Kit, Tm2 ELISA Kit, EDI_309330 ELISA Kit, LOC100101174 ELISA Kit, tpm2 ELISA Kit, tpm2.L ELISA Kit, TPM2 ELISA Kit, Tpm2 ELISA Kit

Background: Alternative name: AMCD1, DA1, TMSB, Arthrogryposis Multiplex Congenital,Distal,Type 1

Gene ID: 22004

UniProt: P58774