ARHGEF7 ELISA Kit

Catalog No. ABIN5674758

Product Details ARHGEF7 ELISA Kit

Target: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.125 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 34 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ARHGEF7

Target: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Alternative Name: Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) (ARHGEF7 Products)

Synonyms: BETA-PIX ELISA Kit, COOL-1 ELISA Kit, COOL1 ELISA Kit, Nbla10314 ELISA Kit, P50 ELISA Kit, P50BP ELISA Kit, P85 ELISA Kit, P85COOL1 ELISA Kit, P85SPR ELISA Kit, PAK3 ELISA Kit, PIXB ELISA Kit, cool1 ELISA Kit, p50 ELISA Kit, p85 ELISA Kit, pak3 ELISA Kit, pixb ELISA Kit, ARHGEF7 ELISA Kit, P85spr ELISA Kit, Pak3bp ELISA Kit, Cool ELISA Kit, PIX ELISA Kit, betaPix ELISA Kit, betaPix-b ELISA Kit, betaPix-c ELISA Kit, cool-1 ELISA Kit, mKIAA0142 ELISA Kit, p85Cool1 ELISA Kit, p85SPR ELISA Kit, arhgef7 ELISA Kit, bbh ELISA Kit, bpix ELISA Kit, im:7142367 ELISA Kit, wu:fc35h08 ELISA Kit, zgc:101775 ELISA Kit, Rho guanine nucleotide exchange factor 7 ELISA Kit, Rho guanine nucleotide exchange factor 7 S homeolog ELISA Kit, ankyrin repeat domain 10 ELISA Kit, Rho guanine nucleotide exchange factor (GEF7) ELISA Kit, Rho guanine nucleotide exchange factor (GEF) 7b ELISA Kit, ARHGEF7 ELISA Kit, arhgef7.S ELISA Kit, arhgef7 ELISA Kit, ANKRD10 ELISA Kit, Arhgef7 ELISA Kit, arhgef7b ELISA Kit

Background: Alternative name: PAK3, BETA-PIX, COOL1, P50, P50BP, P85, P85SPR, PIXB, SH3 Domain-Containing Proline-Rich Protein, PAK-Interacting Exchange Factor Beta

Gene ID: 54126

UniProt: Q9ES28

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation