UROD ELISA Kit

Catalog No. ABIN5674732

Product Details UROD ELISA Kit

Target: UROD (Uroporphyrinogen Decarboxylase (UROD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0062 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for UROD

Target: UROD (Uroporphyrinogen Decarboxylase (UROD))

Abstract: UROD Products

Synonyms: UROD ELISA Kit, pct ELISA Kit, wu:fc43e09 ELISA Kit, PCT ELISA Kit, UPD ELISA Kit, AI323803 ELISA Kit, Uro-d ELISA Kit, porphyrinogen carboxy-lyase ELISA Kit, uroporphyrinogen decarboxylase ELISA Kit, Uroporphyrinogen decarboxylase UroD ELISA Kit, Uroporphyrinogen decarboxylase ELISA Kit, UROD ELISA Kit, urod ELISA Kit, hemE ELISA Kit, uroD ELISA Kit, Cpin_6502 ELISA Kit, Rmar_1187 ELISA Kit, LOC5568261 ELISA Kit, Mrub_1403 ELISA Kit, Arnit_2230 ELISA Kit, Ndas_2830 ELISA Kit, Mesil_2989 ELISA Kit, Trad_0222 ELISA Kit, Weevi_0432 ELISA Kit, Hipma_1229 ELISA Kit, Fluta_0529 ELISA Kit, Marky_1143 ELISA Kit, Halhy_5610 ELISA Kit, Mesop_0899 ELISA Kit, Ccan_20670 ELISA Kit, Urod ELISA Kit

Background: Alternative name: PCT, Uroporphyrinogen III Decarboxylase

Gene ID: 7389

UniProt: P06132