SF3B3 ELISA Kit

Catalog No. ABIN5674722

Product Details SF3B3 ELISA Kit

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0065 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for SF3B3

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Alternative Name: Splicing Factor 3B Subunit 3 (SF3B3) (SF3B3 Products)

Synonyms: GB11574 ELISA Kit, rse1 ELISA Kit, sap130 ELISA Kit, sf3b130 ELISA Kit, staf130 ELISA Kit, sf3b3 ELISA Kit, 1810061H24Rik ELISA Kit, 5730409A01Rik ELISA Kit, AA409318 ELISA Kit, D8Ertd633e ELISA Kit, RSE1 ELISA Kit, SAP130 ELISA Kit, mKIAA0017 ELISA Kit, ik:tdsubc_2b2 ELISA Kit, wu:fb81f05 ELISA Kit, xx:tdsubc_2b2 ELISA Kit, zgc:55440 ELISA Kit, SF3b130 ELISA Kit, STAF130 ELISA Kit, splicing factor 3b subunit 3 ELISA Kit, splicing factor 3B subunit 3 ELISA Kit, splicing factor 3b, subunit 3, 130kDa ELISA Kit, splicing factor 3b subunit 3 L homeolog ELISA Kit, splicing factor 3b, subunit 3 ELISA Kit, SF3B3 ELISA Kit, LOC550935 ELISA Kit, sf3b3 ELISA Kit, Sf3b3 ELISA Kit, LOC100178056 ELISA Kit, sf3b3.L ELISA Kit

Background: Alternative name: RSE1, SAP130, SF3b130, STAF130, Spliceosome-associated protein 130, Pre-mRNA-splicing factor SF3b 130 kDa subunit

Gene ID: 23450

UniProt: Q15393