HRH1 ELISA Kit
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- Target See all HRH1 ELISA Kits
- HRH1 (Histamine Receptor H1 (HRH1))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.312 ng/mL - 20 ng/mL
- Minimum Detection Limit
- 0.312 ng/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 0113 ng/mL
- Components
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Material not included
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product HRH1 ELISA Kit
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Histamine Receptor H1 (HRH1) ELISA Kit
HRH1 Reactivity: Rat Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Histamine Receptor H1 (HRH1) ELISA KitHRH1 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Histamine Receptor H1 (HRH1) ELISA KitHRH1 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target See all HRH1 ELISA Kits
- HRH1 (Histamine Receptor H1 (HRH1))
- Abstract
- HRH1 Products
- Synonyms
- H1-R ELISA Kit, hisH1 ELISA Kit, Bphs ELISA Kit, H1R ELISA Kit, HH1R ELISA Kit, Hir ELISA Kit, HRH1 ELISA Kit, H1 ELISA Kit, Hisr ELISA Kit, histamine receptor H1 ELISA Kit, histamine receptor H 1 ELISA Kit, HRH1 ELISA Kit, Hrh1 ELISA Kit, hrh1 ELISA Kit
- Background
- Alternative name: H1-R, hisH1
- Gene ID
- 15465
- UniProt
- P70174
- Pathways
- Regulation of Carbohydrate Metabolic Process
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