GRIN1/NMDAR1 ELISA Kit

Catalog No. ABIN5674688

Product Details GRIN1/NMDAR1 ELISA Kit

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0122 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Alternative Name: Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) (GRIN1 Products)

Synonyms: GluN1 ELISA Kit, MRD8 ELISA Kit, NMDA1 ELISA Kit, NMDAR1 ELISA Kit, NR1 ELISA Kit, nmda1 ELISA Kit, nmdar1 ELISA Kit, GB19253 ELISA Kit, GRIN1 ELISA Kit, NMD-R1 ELISA Kit, Z16 ELISA Kit, GluRdelta1 ELISA Kit, GluRzeta1 ELISA Kit, M100174 ELISA Kit, Nmdar ELISA Kit, Rgsc174 ELISA Kit, NMDAR1.1 ELISA Kit, zgc:152678 ELISA Kit, glutamate ionotropic receptor NMDA type subunit 1 ELISA Kit, glutamate receptor, ionotropic, N-methyl D-aspartate 1 S homeolog ELISA Kit, NMDA receptor 1 ELISA Kit, G protein-regulated inducer of neurite outgrowth 1 ELISA Kit, glutamate receptor, ionotropic, NMDA1 (zeta 1) ELISA Kit, glutamate receptor, ionotropic, N-methyl D-aspartate 1a ELISA Kit, GRIN1 ELISA Kit, grin1.S ELISA Kit, grin1 ELISA Kit, Grin1 ELISA Kit, Nmdar1 ELISA Kit, Gprin1 ELISA Kit, grin1a ELISA Kit

Background: Alternative name: NMDA1, NMDAR1, NR1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1

Gene ID: 14810

UniProt: P35438

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity