ADORA1 ELISA Kit

Catalog No. ABIN5674664

Product Details ADORA1 ELISA Kit

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0061 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ADORA1

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Abstract: ADORA1 Products

Synonyms: adora1-A ELISA Kit, a1R ELISA Kit, ADORA1 ELISA Kit, LOC606743 ELISA Kit, LOC100222054 ELISA Kit, RDC7 ELISA Kit, A1-AR ELISA Kit, A1AR ELISA Kit, A1R ELISA Kit, AA1R ELISA Kit, AI848715 ELISA Kit, BB176431 ELISA Kit, Ri ELISA Kit, adenosine A1 receptor L homeolog ELISA Kit, adenosine A1 receptor ELISA Kit, adenosine receptor A1 ELISA Kit, adora1.L ELISA Kit, ADORA1 ELISA Kit, adora1 ELISA Kit, LOC100222054 ELISA Kit, Adora1 ELISA Kit

Background: Alternative name: RDC7

Gene ID: 134

UniProt: P30542

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane