ATP1A1 ELISA Kit

Catalog No. ABIN5674661

Product Details ATP1A1 ELISA Kit

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.125 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 26.6 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Alternative Name: ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) (ATP1A1 Products)

Synonyms: Atpa-1 ELISA Kit, BC010319 ELISA Kit, Nkaa1b ELISA Kit, K-ATPase ELISA Kit, Na+K+ATPase ELISA Kit, atp1a1 ELISA Kit, atp1a1a.1 ELISA Kit, atp1a1l1 ELISA Kit, atp[a]1B1 ELISA Kit, cb70 ELISA Kit, wu:fa03c03 ELISA Kit, wu:fb92h10 ELISA Kit, atp1a1l5 ELISA Kit, atp[a]1B2 ELISA Kit, cb707 ELISA Kit, wu:fj34g01 ELISA Kit, zgc:92351 ELISA Kit, ATPase Na+/K+ transporting subunit alpha 1 ELISA Kit, ATPase, Na+/K+ transporting, alpha 1 polypeptide ELISA Kit, ATPase Na+/K+ transporting subunit alpha 1 L homeolog ELISA Kit, ATPase, Na+/K+ transporting, alpha 1a polypeptide, tandem duplicate 1 ELISA Kit, ATPase, Na+/K+ transporting, alpha 1b polypeptide ELISA Kit, ATP1A1 ELISA Kit, Atp1a1 ELISA Kit, atp1a1.L ELISA Kit, atp1a1a.1 ELISA Kit, atp1a1b ELISA Kit

Background: Alternative name: Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1

Gene ID: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process