EIF2A ELISA Kit

Catalog No. ABIN5661958

Product Details EIF2A ELISA Kit

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 5.0 ng/mL - 100 ng/mL

Minimum Detection Limit: 5.0 ng/mL

Application: ELISA

Analytical Method: Quantitative

Sensitivity: 1.0 ng/mL

Components:

• Microtiter plate (96 wells stripwell) - 1
• Enzyme conjugate - 1 vial
• Standard A - 1 vial
• Standard B - 1 vial
• Standard C - 1 vial
• Standard D - 1 vial
• Standard E - 1 vial
• Standard F - 1 vial
• Substrate A - 1 vial
• Substrate B - 1 vial
• Stop solution - 1 vial
• Wash solution - 1 vial
• Balance solution - 1 vial
• Instruction manual - 1

Material not included:

• Precision pipettors and disposable tips to deliver 10-1000 μL. A multi-channel pipette is desirable for large assays.
• 100 mL and 1 L graduated cylinders.
• Distilled or deionized water
• Tubes to prepare sample dilutions.
• Absorbent paper.
• Microplate reader capable of measuring absorbance at 450 nm.
• Centrifuge capable of 3000 x g.
• Microplate washer or washing bottle.
• Incubator (37 °C).
• Data analysis and graphing software.

Application Details

Application Notes:

• The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of samples used in the whole test. Please reserve sufficient amounts of samples in advance.
• Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
• If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
• Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
• Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
• Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: Eukaryotic Translation Initiation Factor 2A (EIF2A Products)

Synonyms: ATEIF2-A2 ELISA Kit, EIF2-A2 ELISA Kit, eukaryotic translation initiation factor 2 alpha subunit ELISA Kit, EIF-2A ELISA Kit, MST089 ELISA Kit, MSTP004 ELISA Kit, MSTP089 ELISA Kit, D030048D22 ELISA Kit, D3Ertd194e ELISA Kit, im:7145971 ELISA Kit, zgc:110460 ELISA Kit, DDBDRAFT_0185936 ELISA Kit, DDBDRAFT_0234177 ELISA Kit, DDB_0185936 ELISA Kit, DDB_0234177 ELISA Kit, Eif2a ELISA Kit, eif2a ELISA Kit, eif2alpha ELISA Kit, EIF2A ELISA Kit, CG9946 ELISA Kit, Dmel\\CG9946 ELISA Kit, Eif2alpha ELISA Kit, IF2A_DROME ELISA Kit, M(1)14C ELISA Kit, M(1)19-153 ELISA Kit, M(1)8e3-10 ELISA Kit, deIF2alpha ELISA Kit, eIF-2 ELISA Kit, eIF-2 a ELISA Kit, eIF-2 alpha ELISA Kit, eIF2 alpha ELISA Kit, eIF2-alpha ELISA Kit, eIF2a ELISA Kit, eIF2alpha ELISA Kit, eiF-2alpha ELISA Kit, elF2alpha ELISA Kit, l(1)14Cf ELISA Kit, l(1)19-153 ELISA Kit, eukaryotic translation initiation factor 2 alpha subunit ELISA Kit, eukaryotic translation initiation factor 2A ELISA Kit, eukaryotic translation initiation factor 2A, 65kDa ELISA Kit, eukaryotic translation initiation factor 2A S homeolog ELISA Kit, eukaryotic translation initiation factor ELISA Kit, eukaryotic translation Initiation Factor 2alpha ELISA Kit, EIF2 ALPHA ELISA Kit, EIF2A ELISA Kit, Eif2a ELISA Kit, eif2a ELISA Kit, eIF2a ELISA Kit, eif2a.S ELISA Kit, eif2A ELISA Kit, eIF-2alpha ELISA Kit

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly