ZEB2 CLIA Kit

Catalog No. ABIN5660076

Product Details ZEB2 CLIA Kit

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Zinc Finger Homeobox Protein 1B (ZFHX1B). No significant cross-reactivity or interference between Zinc Finger Homeobox Protein 1B (ZFHX1B) and analogues was observed.

Sensitivity: 6.45 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Zinc Finger Homeobox Protein 1B (ZFHX1B). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Zinc Finger Homeobox Protein 1B (ZFHX1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Zinc Finger Homeobox Protein 1B (ZFHX1B) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Alternative Name: Zinc Finger Homeobox Protein 1B (ZEB2 Products)

Synonyms: ZFHX1B CLIA Kit, Zfhx1b CLIA Kit, HSPC082 CLIA Kit, SIP-1 CLIA Kit, SIP1 CLIA Kit, SMADIP1 CLIA Kit, 9130203F04Rik CLIA Kit, D130016B08Rik CLIA Kit, Zfx1b CLIA Kit, Zfxh1b CLIA Kit, mKIAA0569 CLIA Kit, XSip1 CLIA Kit, sip1 CLIA Kit, zfhx1b CLIA Kit, si:dkey-52o2.2 CLIA Kit, sip1a CLIA Kit, wu:fc92g08 CLIA Kit, sip1b CLIA Kit, zinc finger E-box binding homeobox 2 CLIA Kit, zinc finger E-box binding homeobox 2 S homeolog CLIA Kit, zinc finger homeobox protein CLIA Kit, zinc finger E-box binding homeobox 2a CLIA Kit, zinc finger E-box binding homeobox 2b CLIA Kit, ZEB2 CLIA Kit, Zeb2 CLIA Kit, zeb2.S CLIA Kit, zeb2 CLIA Kit, zeb2a CLIA Kit, zeb2b CLIA Kit

Background:

Gene Name: Zinc Finger Homeobox Protein 1B

Gene Aliases: ZEB2, SIP1, SIP-1, SMADIP1, SIP1, Zinc Finger E-Box Binding Homeobox 2, SMAD Interacting Protein 1

Gene ID: 24136

UniProt: Q9R0G7

Pathways: Tube Formation