YY1 ELISA Kit

Catalog No. ABIN5660075

Product Details YY1 ELISA Kit

Target: YY1 (YY1 Transcription Factor (YY1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of YY1 Transcription Factor (YY1). No significant cross-reactivity or interference between YY1 Transcription Factor (YY1) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to YY1 Transcription Factor (YY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to YY1 Transcription Factor (YY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain YY1 Transcription Factor (YY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of YY1 Transcription Factor (YY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level YY1 Transcription Factor (YY1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level YY1 Transcription Factor (YY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for YY1

Target: YY1 (YY1 Transcription Factor (YY1))

Alternative Name: YY1 Transcription Factor (YY1 Products)

Synonyms: yy1-a ELISA Kit, FIII ELISA Kit, MGC83714 ELISA Kit, yy1l ELISA Kit, zgc:64207 ELISA Kit, YY1 ELISA Kit, LOC494431 ELISA Kit, xyy1 ELISA Kit, delta ELISA Kit, nf-e1 ELISA Kit, ucrbp ELISA Kit, yin-yang-1 ELISA Kit, LOC100304682 ELISA Kit, ino80s ELISA Kit, yy1 ELISA Kit, DELTA ELISA Kit, INO80S ELISA Kit, NF-E1 ELISA Kit, UCRBP ELISA Kit, YIN-YANG-1 ELISA Kit, AW488674 ELISA Kit, fb59g10 ELISA Kit, wu:fa16g07 ELISA Kit, wu:fb59g10 ELISA Kit, YY1 transcription factor L homeolog ELISA Kit, YY1 transcription factor b ELISA Kit, YY1 transcription factor ELISA Kit, transcriptional repressor protein YY1 ELISA Kit, YY1 transcription factor S homeolog ELISA Kit, YY1 transcription factor a ELISA Kit, YY2 transcription factor ELISA Kit, yy1.L ELISA Kit, yy1b ELISA Kit, YY1 ELISA Kit, yy1 ELISA Kit, LOC100304682 ELISA Kit, yy1.S ELISA Kit, Yy1 ELISA Kit, yy1a ELISA Kit, YY2 ELISA Kit

Background:

Gene Name: YY1 Transcription Factor

Gene Aliases: NF-E1, DELTA, UCRBP, YIN-YANG-1, INO80S, INO80 Complex Subunit S, Yin and Yang 1 Protein, Delta transcription factor, INO80 complex subunit S