ERCC5 CLIA Kit

Catalog No. ABIN5660071

Product Details ERCC5 CLIA Kit

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Xeroderma Pigmentosum, Complementation Group G (XPG). No significant cross-reactivity or interference between Xeroderma Pigmentosum, Complementation Group G (XPG) and analogues was observed.

Sensitivity: 5.51 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Xeroderma Pigmentosum, Complementation Group G (XPG) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group G (XPG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group G (XPG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Alternative Name: Xeroderma Pigmentosum, Complementation Group G (ERCC5 Products)

Synonyms: COFS3 CLIA Kit, ERCM2 CLIA Kit, UVDR CLIA Kit, XPG CLIA Kit, XPGC CLIA Kit, cofs3 CLIA Kit, ercm2 CLIA Kit, uvdr CLIA Kit, xpg CLIA Kit, xpgc CLIA Kit, Xpg CLIA Kit, ERCC excision repair 5, endonuclease CLIA Kit, excision repair cross-complementation group 5 L homeolog CLIA Kit, excision repair cross-complementing rodent repair deficiency, complementation group 5 CLIA Kit, ERCC5 CLIA Kit, ercc5.L CLIA Kit, Ercc5 CLIA Kit

Background:

Gene Name: Xeroderma Pigmentosum, Complementation Group G

Gene Aliases: ERCM2, ERCC5, UVDR, XPGC, Excision Repair Cross-Complementing Rodent Repair Deficiency 5, Cockayne Syndrome, DNA repair protein complementing XP-G cells

Gene ID: 2073

UniProt: P28715

Pathways: DNA Damage Repair