XRCC6 ELISA Kit
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- Target See all XRCC6 ELISA Kits
- XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.312 ng/mL - 20 ng/mL
- Minimum Detection Limit
- 0.312 ng/mL
- Application
- ELISA
- Sample Type
- Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of X-Ray Repair Cross Complementing 6 (XRCC6). No significant cross-reactivity or interference between X-Ray Repair Cross Complementing 6 (XRCC6) and analogues was observed.
- Sensitivity
- 0.107 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain X-Ray Repair Cross Complementing 6 (XRCC6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of X-Ray Repair Cross Complementing 6 (XRCC6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level X-Ray Repair Cross Complementing 6 (XRCC6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level X-Ray Repair Cross Complementing 6 (XRCC6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all XRCC6 ELISA Kits
- XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))
- Alternative Name
- X-Ray Repair Cross Complementing 6 (XRCC6 Products)
- Synonyms
- CTC75 ELISA Kit, CTCBF ELISA Kit, G22P1 ELISA Kit, KU70 ELISA Kit, ML8 ELISA Kit, TLAA ELISA Kit, 70kDa ELISA Kit, G22p1 ELISA Kit, Ku70 ELISA Kit, Kup70 ELISA Kit, X-ray repair cross complementing 6 ELISA Kit, ATP-dependent DNA helicase II, 70 kDa subunit ELISA Kit, X-ray repair complementing defective repair in Chinese hamster cells 6 L homeolog ELISA Kit, X-ray repair complementing defective repair in Chinese hamster cells 6 ELISA Kit, XRCC6 ELISA Kit, Bm1_41430 ELISA Kit, xrcc6.L ELISA Kit, Xrcc6 ELISA Kit
- Background
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Gene Name: X-Ray Repair Cross Complementing 6
Gene Aliases: Ku70, CTC75, CTCBF, G22P1, ML8, TLAA, Lupus Ku Autoantigen Protein P70, ATP-Dependent DNA Helicase 2 Subunit 1, CTC box-binding factor 75 kDa, Thyroid-lupus autoantigen
- Gene ID
- 14375
- UniProt
- P23475
- Pathways
- DNA Damage Repair
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