VKORC1 ELISA Kit

Catalog No. ABIN5659967

Product Details VKORC1 ELISA Kit

Target: VKORC1 (Vitamin K Epoxide Reductase Complex, Subunit 1 (VKORC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1). No significant cross-reactivity or interference between Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) and analogues was observed.

Sensitivity: 0.57 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for VKORC1

Target: VKORC1 (Vitamin K Epoxide Reductase Complex, Subunit 1 (VKORC1))

Alternative Name: Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1 Products)

Synonyms: MGC89620 ELISA Kit, BP1071 ELISA Kit, CG33544 ELISA Kit, Dmel\\CG33544 ELISA Kit, HDC06808 ELISA Kit, VKORC1 ELISA Kit, EDTP308 ELISA Kit, IMAGE3455200 ELISA Kit, MST134 ELISA Kit, MST576 ELISA Kit, VKCFD2 ELISA Kit, VKOR ELISA Kit, D7Wsu86e ELISA Kit, vitamin K epoxide reductase complex, subunit 1 ELISA Kit, vitamin K epoxide reductase complex subunit 1 ELISA Kit, Vitamin-K epoxide reductase ELISA Kit, VKORC1 ELISA Kit, vkorc1 ELISA Kit, Vkor ELISA Kit, Vkorc1 ELISA Kit

Background:

Gene Name: Vitamin K Epoxide Reductase Complex Subunit 1

Gene Aliases: VKCFD2, VKOR, Vitamin K Dependent Clotting Factors Deficiency 2, Vitamin K1 2,3-epoxide reductase subunit 1