VSNL1 ELISA Kit

Catalog No. ABIN5659949

Product Details VSNL1 ELISA Kit

Target: VSNL1 (Visinin-Like 1 (VSNL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cerebrospinal Fluid, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Visinin Like Protein 1 (VSNL1). No significant cross-reactivity or interference between Visinin Like Protein 1 (VSNL1) and analogues was observed.

Sensitivity: 0.062 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visinin Like Protein 1 (VSNL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visinin Like Protein 1 (VSNL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visinin Like Protein 1 (VSNL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Visinin Like Protein 1 (VSNL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visinin Like Protein 1 (VSNL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visinin Like Protein 1 (VSNL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for VSNL1

Target: VSNL1 (Visinin-Like 1 (VSNL1))

Alternative Name: Visinin Like Protein 1 (VSNL1 Products)

Synonyms: HLP3 ELISA Kit, HPCAL3 ELISA Kit, HUVISL1 ELISA Kit, VILIP ELISA Kit, VILIP-1 ELISA Kit, Nvp1 ELISA Kit, Ratnvp1 ELISA Kit, Vnsl1 ELISA Kit, MGC132057 ELISA Kit, MGC146004 ELISA Kit, VSNL1 ELISA Kit, si:dkey-15f12.11 ELISA Kit, si:dkey-30j22.11 ELISA Kit, zgc:103644 ELISA Kit, hlp3 ELISA Kit, vilip ELISA Kit, hpcal3 ELISA Kit, huvisl1 ELISA Kit, vilip-1 ELISA Kit, visinin like 1 ELISA Kit, visinin-like 1 ELISA Kit, visinin like 1 S homeolog ELISA Kit, visinin-like 1a ELISA Kit, visinin like 1 L homeolog ELISA Kit, VSNL1 ELISA Kit, Vsnl1 ELISA Kit, vsnl1.S ELISA Kit, vsnl1 ELISA Kit, vsnl1a ELISA Kit, vsnl1.L ELISA Kit

Background:

Gene Name: Visinin Like Protein 1

Gene Aliases: HLP3, VISL1, HPCAL3, HUVISL1, VILIP, VILIP-1, VLP-1, Hippocalcin Like Protein 3

Gene ID: 7447

UniProt: P62760