UROD ELISA Kit

Catalog No. ABIN5659792

Product Details UROD ELISA Kit

Target: UROD (Uroporphyrinogen Decarboxylase (UROD))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Uroporphyrinogen Decarboxylase (UROD). No significant cross-reactivity or interference between Uroporphyrinogen Decarboxylase (UROD) and analogues was observed.

Sensitivity: 0.117 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uroporphyrinogen Decarboxylase (UROD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uroporphyrinogen Decarboxylase (UROD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uroporphyrinogen Decarboxylase (UROD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Uroporphyrinogen Decarboxylase (UROD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uroporphyrinogen Decarboxylase (UROD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uroporphyrinogen Decarboxylase (UROD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for UROD

Target: UROD (Uroporphyrinogen Decarboxylase (UROD))

Alternative Name: Uroporphyrinogen Decarboxylase (UROD Products)

Synonyms: UROD ELISA Kit, pct ELISA Kit, wu:fc43e09 ELISA Kit, PCT ELISA Kit, UPD ELISA Kit, AI323803 ELISA Kit, Uro-d ELISA Kit, porphyrinogen carboxy-lyase ELISA Kit, uroporphyrinogen decarboxylase ELISA Kit, Uroporphyrinogen decarboxylase UroD ELISA Kit, Uroporphyrinogen decarboxylase ELISA Kit, UROD ELISA Kit, urod ELISA Kit, hemE ELISA Kit, uroD ELISA Kit, Cpin_6502 ELISA Kit, Rmar_1187 ELISA Kit, LOC5568261 ELISA Kit, Mrub_1403 ELISA Kit, Arnit_2230 ELISA Kit, Ndas_2830 ELISA Kit, Mesil_2989 ELISA Kit, Trad_0222 ELISA Kit, Weevi_0432 ELISA Kit, Hipma_1229 ELISA Kit, Fluta_0529 ELISA Kit, Marky_1143 ELISA Kit, Halhy_5610 ELISA Kit, Mesop_0899 ELISA Kit, Ccan_20670 ELISA Kit, Urod ELISA Kit

Background:

Gene Name: Uroporphyrinogen Decarboxylase

Gene Aliases: PCT, Uroporphyrinogen III Decarboxylase

UniProt: P32362