UCP1 ELISA Kit

Catalog No. ABIN5659758

Product Details UCP1 ELISA Kit

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Uncoupling Protein 1, Mitochondrial (UCP1). No significant cross-reactivity or interference between Uncoupling Protein 1, Mitochondrial (UCP1) and analogues was observed.

Sensitivity: 0.114 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uncoupling Protein 1, Mitochondrial (UCP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uncoupling Protein 1, Mitochondrial (UCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uncoupling Protein 1, Mitochondrial (UCP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Uncoupling Protein 1, Mitochondrial (UCP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uncoupling Protein 1, Mitochondrial (UCP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uncoupling Protein 1, Mitochondrial (UCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for UCP1

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: Uncoupling Protein 1, Mitochondrial (UCP1 Products)

Synonyms: ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1 ELISA Kit, ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1 ELISA Kit, ATPUMP1 ELISA Kit, ATUCP1 ELISA Kit, UCP ELISA Kit, UCP1 ELISA Kit, UNCOUPLING PROTEIN 1 ELISA Kit, plant uncoupling mitochondrial protein 1 ELISA Kit, ucp ELISA Kit, slc25a7 ELISA Kit, SLC25A7 ELISA Kit, AI385626 ELISA Kit, Slc25a7 ELISA Kit, Ucp ELISA Kit, Ucpa ELISA Kit, Uncp ELISA Kit, plant uncoupling mitochondrial protein 1 ELISA Kit, uncoupling protein 1 ELISA Kit, uncoupling protein 1 L homeolog ELISA Kit, uncoupling protein 1 (mitochondrial, proton carrier) ELISA Kit, PUMP1 ELISA Kit, UCP1 ELISA Kit, ucp1.L ELISA Kit, ucp1 ELISA Kit, Ucp1 ELISA Kit

Background:

Gene Name: Uncoupling Protein 1, Mitochondrial

Gene Aliases: SLC25A7, UCP, Thermogenin, Solute carrier family 25 member 7

Gene ID: 24860

UniProt: P04633

Pathways: Proton Transport, Brown Fat Cell Differentiation