UGGT1 ELISA Kit

Catalog No. ABIN5659754

Product Details UGGT1 ELISA Kit

Target: UGGT1 (UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1). No significant cross-reactivity or interference between UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1) and analogues was observed.

Sensitivity: 0.113 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for UGGT1

Target: UGGT1 (UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1))

Alternative Name: UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1 Products)

Synonyms: UGCGL1 ELISA Kit, ugcgl1 ELISA Kit, uggt2 ELISA Kit, 0910001L17Rik ELISA Kit, A930007H10Rik ELISA Kit, AA589501 ELISA Kit, AI414429 ELISA Kit, AI448372 ELISA Kit, C820010P03Rik ELISA Kit, GT ELISA Kit, UGT1 ELISA Kit, Ugcgl1 ELISA Kit, Uggt ELISA Kit, rUGT1 ELISA Kit, HUGT1 ELISA Kit, EMS-mutagenized bri1 suppressor 1 ELISA Kit, F3I17.13 ELISA Kit, F3I17_13 ELISA Kit, PRIORITY IN SWEET LIFE 2 ELISA Kit, PSL2 ELISA Kit, UDP-GLUCOSE:GLYCOPROTEIN GLUCOSYLTRANSFERASE ELISA Kit, UGGT ELISA Kit, UDP-glucose glycoprotein glucosyltransferase 1 ELISA Kit, UDP-glucose glycoprotein glucosyltransferase 1 L homeolog ELISA Kit, UDP-glucose:glycoprotein glucosyltransferase 1 ELISA Kit, EMS-MUTAGENIZED BRI1 SUPPRESSOR 1 ELISA Kit, UGGT1 ELISA Kit, uggt1.L ELISA Kit, uggt1 ELISA Kit, LOC100545947 ELISA Kit, Uggt1 ELISA Kit, EBS1 ELISA Kit

Background:

Gene Name: UDP-Glucose Glycoprotein Glucosyltransferase 1

Gene Aliases: UGTR, HUGT1, GT, UGCGL1, UGGT, UGT1, UDP-glucose ceramide glucosyltransferase-like 1

Gene ID: 56886

UniProt: Q9NYU2

Pathways: Activation of Innate immune Response