ube3a ELISA Kit

Catalog No. ABIN5659743

Product Details ube3a ELISA Kit

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Protein Ligase E3A (UBE3A). No significant cross-reactivity or interference between Ubiquitin Protein Ligase E3A (UBE3A) and analogues was observed.

Sensitivity: 0.114 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3A (UBE3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Protein Ligase E3A (UBE3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Protein Ligase E3A (UBE3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Protein Ligase E3A (UBE3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Protein Ligase E3A (UBE3A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Protein Ligase E3A (UBE3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Alternative Name: Ubiquitin Protein Ligase E3A (ube3a Products)

Synonyms: ANCR ELISA Kit, AS ELISA Kit, E6-AP ELISA Kit, EPVE6AP ELISA Kit, HPVE6A ELISA Kit, DDBDRAFT_0188760 ELISA Kit, DDBDRAFT_0302447 ELISA Kit, DDB_0188760 ELISA Kit, DDB_0302447 ELISA Kit, ube3a ELISA Kit, im:7140733 ELISA Kit, zgc:92173 ELISA Kit, 4732496B02 ELISA Kit, 5830462N02Rik ELISA Kit, A130086L21Rik ELISA Kit, Hpve6a ELISA Kit, mKIAA4216 ELISA Kit, As ELISA Kit, CG6190 ELISA Kit, Dmel\\CG6190 ELISA Kit, Dube3a ELISA Kit, UBE3A ELISA Kit, dUBE3A ELISA Kit, das ELISA Kit, dube3A ELISA Kit, dube3a ELISA Kit, MGC69536 ELISA Kit, UME3A ELISA Kit, ubiquitin protein ligase E3A ELISA Kit, ubiquitin-protein ligase E3A ELISA Kit, Ubiquitin protein ligase E3A ELISA Kit, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) S homeolog ELISA Kit, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) ELISA Kit, UBE3A ELISA Kit, ube3a ELISA Kit, LOC100194730 ELISA Kit, Ube3a ELISA Kit, ube3a.S ELISA Kit, Eint_040430 ELISA Kit

Background:

Gene Name: Ubiquitin Protein Ligase E3A

Gene Aliases: ANCR, AS, E6-AP, EPVE6AP, HPVE6A, Papilloma Virus E6-Associated,Angelman Syndrome, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54

Gene ID: 7337

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway