UBE2L3 CLIA Kit

Catalog No. ABIN5659736

Product Details UBE2L3 CLIA Kit

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). No significant cross-reactivity or interference between Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) and analogues was observed.

Sensitivity: 5.28 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Alternative Name: Ubiquitin Conjugating Enzyme E2L3 (UBE2L3 Products)

Synonyms: E2-F1 CLIA Kit, L-UBC CLIA Kit, UBCH7 CLIA Kit, UbcM4 CLIA Kit, C79827 CLIA Kit, Ubce7 CLIA Kit, ube2l3 CLIA Kit, wu:fc22h11 CLIA Kit, zgc:86727 CLIA Kit, UBE2L3 CLIA Kit, ube2l3l CLIA Kit, wu:fb38h03 CLIA Kit, ubiquitin conjugating enzyme E2 L3 CLIA Kit, ubiquitin-conjugating enzyme E2L 3 CLIA Kit, ubiquitin-conjugating enzyme E2L 3a CLIA Kit, ubiquitin conjugating enzyme E2 L3 S homeolog CLIA Kit, ubiquitin-conjugating enzyme E2L 3b CLIA Kit, UBE2L3 CLIA Kit, Ube2l3 CLIA Kit, ube2l3a CLIA Kit, ube2l3.S CLIA Kit, ube2l3b CLIA Kit

Background:

Gene Name: Ubiquitin Conjugating Enzyme E2L3

Gene Aliases: L-UBC, E2-F1, UBCH7, UbcM4, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3

Gene ID: 7332

UniProt: P68036