UQCRC2 ELISA Kit

Catalog No. ABIN5659722

Product Details UQCRC2 ELISA Kit

Target: UQCRC2 (Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2). No significant cross-reactivity or interference between Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for UQCRC2

Target: UQCRC2 (Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2))

Alternative Name: Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2 Products)

Synonyms: MC3DN5 ELISA Kit, QCR2 ELISA Kit, UQCR2 ELISA Kit, fi06a11 ELISA Kit, uqcrc2 ELISA Kit, wu:fb64g04 ELISA Kit, wu:fi06a11 ELISA Kit, fb33d01 ELISA Kit, fc43c05 ELISA Kit, wu:fb33d01 ELISA Kit, wu:fc43c05 ELISA Kit, zgc:92453 ELISA Kit, MGC80228 ELISA Kit, MGC130698 ELISA Kit, Uqcrc2 ELISA Kit, Afu5g04210 ELISA Kit, AO090102000625 ELISA Kit, 1500004O06Rik ELISA Kit, UQCRC2 ELISA Kit, ubiquinol-cytochrome c reductase core protein 2 ELISA Kit, ubiquinol cytochrome c reductase core protein 2 ELISA Kit, ubiquinol-cytochrome c reductase core protein 2b ELISA Kit, ubiquinol-cytochrome c reductase core protein II ELISA Kit, ubiquinol-cytochrome c reductase core protein 2a ELISA Kit, ubiquinol-cytochrome c reductase core protein 2 L homeolog ELISA Kit, cytochrome b-c1 complex subunit 2, mitochondrial ELISA Kit, ubiquinol-cytochrome C reductase complex core protein 2 ELISA Kit, ubiquinol--cytochrome-c reductase subunit 2 ELISA Kit, UQCRC2 ELISA Kit, Uqcrc2 ELISA Kit, uqcrc2b ELISA Kit, uqcrc2a ELISA Kit, uqcrc2.L ELISA Kit, uqcrc2 ELISA Kit, LOC100026672 ELISA Kit, LOC100389097 ELISA Kit, LOC100456655 ELISA Kit, LOC100592631 ELISA Kit, AFUA_5G04210 ELISA Kit, ANI_1_872084 ELISA Kit, AOR_1_1048134 ELISA Kit, QCR2 ELISA Kit, LOC100861263 ELISA Kit

Background:

Gene Name: Ubiquinol Cytochrome C Reductase Core Protein II

Gene Aliases: QCR2, UQCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Complex III subunit 2, Core protein II