TWSG1 ELISA Kit

Catalog No. ABIN5659703

Product Details TWSG1 ELISA Kit

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Twisted Gastrulation Protein Homolog 1 (TWSG1). No significant cross-reactivity or interference between Twisted Gastrulation Protein Homolog 1 (TWSG1) and analogues was observed.

Sensitivity: 0.122 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Twisted Gastrulation Protein Homolog 1 (TWSG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Twisted Gastrulation Protein Homolog 1 (TWSG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Twisted Gastrulation Protein Homolog 1 (TWSG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Twisted Gastrulation Protein Homolog 1 (TWSG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Twisted Gastrulation Protein Homolog 1 (TWSG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Twisted Gastrulation Protein Homolog 1 (TWSG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TWSG1

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Alternative Name: Twisted Gastrulation Protein Homolog 1 (TWSG1 Products)

Synonyms: TWSG1 ELISA Kit, TSG ELISA Kit, DKFZp469J051 ELISA Kit, 1810013J15Rik ELISA Kit, 9030422N06Rik ELISA Kit, AW552143 ELISA Kit, D17Ertd403e ELISA Kit, Tsg ELISA Kit, Twg ELISA Kit, fi23f01 ELISA Kit, tsg ELISA Kit, tsga ELISA Kit, twsg1 ELISA Kit, wu:fi23f01 ELISA Kit, twsg ELISA Kit, tsg1 ELISA Kit, tsgb ELISA Kit, ztsg1 ELISA Kit, twisted gastrulation BMP signaling modulator 1 ELISA Kit, twisted gastrulation homolog 1 (Drosophila) ELISA Kit, twisted gastrulation BMP signaling modulator 1a ELISA Kit, twisted gastrulation BMP signaling modulator 1 S homeolog ELISA Kit, twisted gastrulation BMP signaling modulator 1 L homeolog ELISA Kit, twisted gastrulation BMP signaling modulator 1b ELISA Kit, TWSG1 ELISA Kit, Twsg1 ELISA Kit, twsg1a ELISA Kit, twsg1.S ELISA Kit, twsg1.L ELISA Kit, twsg1b ELISA Kit

Background:

Gene Name: Twisted Gastrulation Protein Homolog 1

Gene Aliases: TSG

Gene ID: 57045

UniProt: Q9GZX9