TWIST1 ELISA Kit

Catalog No. ABIN5659701

Product Details TWIST1 ELISA Kit

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Twist Transcription Factor (TWIST). No significant cross-reactivity or interference between Twist Transcription Factor (TWIST) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Twist Transcription Factor (TWIST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Twist Transcription Factor (TWIST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Twist Transcription Factor (TWIST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Twist Transcription Factor (TWIST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Twist Transcription Factor (TWIST) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Twist Transcription Factor (TWIST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TWIST1

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Alternative Name: Twist Transcription Factor (TWIST1 Products)

Synonyms: scs ELISA Kit, Xtwi ELISA Kit, acs3 ELISA Kit, X-twi ELISA Kit, bpes2 ELISA Kit, bpes3 ELISA Kit, twist ELISA Kit, Xtwist ELISA Kit, MGC69439 ELISA Kit, twist1 ELISA Kit, ACS3 ELISA Kit, BPES2 ELISA Kit, BPES3 ELISA Kit, CRS1 ELISA Kit, SCS ELISA Kit, TWIST ELISA Kit, bHLHa38 ELISA Kit, AA960487 ELISA Kit, M-Twist ELISA Kit, Pde ELISA Kit, Ska10 ELISA Kit, Ska ELISA Kit, Twist ELISA Kit, pdt ELISA Kit, cTwist ELISA Kit, Twist1b ELISA Kit, twist-1 ELISA Kit, wu:fd04c01 ELISA Kit, zgc:101115 ELISA Kit, twist family bHLH transcription factor 1 ELISA Kit, twist family bHLH transcription factor 1 S homeolog ELISA Kit, twist basic helix-loop-helix transcription factor 1 ELISA Kit, twist homolog 1 (Drosophila) ELISA Kit, twist family bHLH transcription factor 1a ELISA Kit, TWIST1 ELISA Kit, twist1 ELISA Kit, twist1.S ELISA Kit, Twist1 ELISA Kit, twist1a ELISA Kit

Background:

Gene Name: Twist Transcription Factor

Gene Aliases: ACS3, BPES2, BPES3, SCS, Class A basic helix-loop-helix protein 38, Acrocephalosyndactyly 3, Saethre-Chotzen Syndrome, Blepharophimosis,Epicanthus Inversus And Ptosis 3

Pathways: p53 Signaling, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity