TUBB ELISA Kit

Catalog No. ABIN5659603

Product Details TUBB ELISA Kit

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Rat, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Tubulin Beta (TUBb). No significant cross-reactivity or interference between Tubulin Beta (TUBb) and analogues was observed.

Sensitivity: 12.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tubulin Beta (TUBb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tubulin Beta (TUBb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tubulin Beta (TUBb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tubulin Beta (TUBb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tubulin Beta (TUBb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tubulin Beta (TUBb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: Tubulin Beta (TUBB Products)

Synonyms: M40 ELISA Kit, OK/SW-cl.56 ELISA Kit, TUBB1 ELISA Kit, TUBB5 ELISA Kit, XLOT ELISA Kit, m40 ELISA Kit, ok/sw-cl.56 ELISA Kit, tubb1 ELISA Kit, tubb5 ELISA Kit, TUBB2A ELISA Kit, TUBB2B ELISA Kit, TUBB ELISA Kit, 1t ELISA Kit, B1t ELISA Kit, BETA 56D ELISA Kit, CG9277 ELISA Kit, DTB2 ELISA Kit, Dmbeta1 ELISA Kit, Dmel\\CG9277 ELISA Kit, T ELISA Kit, Tub ELISA Kit, Tubulin ELISA Kit, beta ELISA Kit, beta-Tub ELISA Kit, beta-Tub56D ELISA Kit, beta-tub ELISA Kit, beta-tubulin56D ELISA Kit, beta1 ELISA Kit, beta1-Tubulin ELISA Kit, beta1-tub ELISA Kit, beta1Tub ELISA Kit, beta1t ELISA Kit, beta1tub ELISA Kit, beta56D ELISA Kit, betaTub ELISA Kit, betaTub1 ELISA Kit, beta[[1]] tubulin ELISA Kit, beta[[1]]-tubulin ELISA Kit, betatub(56D) ELISA Kit, 143391_i_at ELISA Kit, 3t ELISA Kit, B3t ELISA Kit, BETA 60D ELISA Kit, CG3401 ELISA Kit, D.m.BETA-60D ELISA Kit, DTB3 ELISA Kit, Dmbeta3 ELISA Kit, Dmel\\CG3401 ELISA Kit, Tub60D ELISA Kit, beta-Tub60D ELISA Kit, beta-Tub6D ELISA Kit, beta3 ELISA Kit, beta3 TU ELISA Kit, beta3-Tub ELISA Kit, beta3-tubulin ELISA Kit, beta3TUB ELISA Kit, beta3Tub ELISA Kit, beta3t ELISA Kit, beta60C ELISA Kit, betaTub3 ELISA Kit, betaTub60C ELISA Kit, beta[[3]] tubulin ELISA Kit, beta[[3]]-Tub ELISA Kit, beta[[3]]-tubulin ELISA Kit, betatub60D ELISA Kit, p50 ELISA Kit, p50/tubulin ELISA Kit, p53 ELISA Kit, p53/tubulin ELISA Kit, 2t ELISA Kit, B2t ELISA Kit, BETA 85D ELISA Kit, BETA2 ELISA Kit, CG9359 ELISA Kit, D.m.BETA-85D ELISA Kit, DTB4 ELISA Kit, Dmbeta2 ELISA Kit, Dmel\\CG9359 ELISA Kit, beta(2)Tu ELISA Kit, beta(2)Tub ELISA Kit, beta-Tub85D ELISA Kit, beta-tub85D ELISA Kit, beta2 ELISA Kit, beta2-tub ELISA Kit, beta2t ELISA Kit, beta2tub ELISA Kit, beta85D ELISA Kit, betaTub2 ELISA Kit, beta[[2]]-tubulin ELISA Kit, ms(3)KK[D] ELISA Kit, 4t ELISA Kit, B4t ELISA Kit, BETA 98B ELISA Kit, CG4869 ELISA Kit, DTB1 ELISA Kit, Dmbeta4 ELISA Kit, Dmel\\CG4869 ELISA Kit, beta-Tub97EF ELISA Kit, beta4 ELISA Kit, beta4t ELISA Kit, beta97F ELISA Kit, betaTub4 ELISA Kit, betaTub98BC ELISA Kit, betaTub98C ELISA Kit, Tub1 ELISA Kit, bmtub1 ELISA Kit, Tub3 ELISA Kit, bmtub3 ELISA Kit, Tub2 ELISA Kit, bmtub2 ELISA Kit, Tub4 ELISA Kit, bmtub4 ELISA Kit, LOC100101153 ELISA Kit, tubulin beta class I ELISA Kit, putative cobalt-zinc-cadmium outer membrane resistance protein ELISA Kit, Tubulin beta chain ELISA Kit, tubulin beta-2A chain ELISA Kit, beta-Tubulin at 56D ELISA Kit, beta-Tubulin at 60D ELISA Kit, beta-Tubulin at 85D ELISA Kit, beta-Tubulin at 97EF ELISA Kit, beta-tubulin ELISA Kit, tubulin beta 6 class V ELISA Kit, beta tubulin ELISA Kit, tubulin beta class I L homeolog ELISA Kit, tubulin, beta 5 class I ELISA Kit, TUBB ELISA Kit, czcC ELISA Kit, tbb-6 ELISA Kit, tubb ELISA Kit, LOC462399 ELISA Kit, betaTub56D ELISA Kit, betaTub60D ELISA Kit, betaTub85D ELISA Kit, betaTub97EF ELISA Kit, Tub1 ELISA Kit, Tub3 ELISA Kit, Tub2 ELISA Kit, Tub4 ELISA Kit, TVAG_525430 ELISA Kit, TVAG_062880 ELISA Kit, TVAG_456920 ELISA Kit, LOC100101153 ELISA Kit, TUBB6 ELISA Kit, Tb927.1.2330 ELISA Kit, Tb927.1.2350 ELISA Kit, Tb927.1.2370 ELISA Kit, Tb927.1.2390 ELISA Kit, tubb.L ELISA Kit, Tubb ELISA Kit, LOC693049 ELISA Kit, LOC443015 ELISA Kit, Tubb5 ELISA Kit

Background:

Gene Name: Tubulin Beta

Gene Aliases: TUB-B, TUBB5, TUBB1, M40

Pathways: Microtubule Dynamics, M Phase