WARS ELISA Kit

Catalog No. ABIN5659601

Product Details WARS ELISA Kit

Target: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Tryptophanyl tRNA Synthetase (WARS). No significant cross-reactivity or interference between Tryptophanyl tRNA Synthetase (WARS) and analogues was observed.

Sensitivity: 0.064 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptophanyl tRNA Synthetase (WARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tryptophanyl tRNA Synthetase (WARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptophanyl tRNA Synthetase (WARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tryptophanyl tRNA Synthetase (WARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptophanyl tRNA Synthetase (WARS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptophanyl tRNA Synthetase (WARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for WARS

Target: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Alternative Name: Tryptophanyl tRNA Synthetase (WARS Products)

Synonyms: GAMMA-2 ELISA Kit, IFI53 ELISA Kit, IFP53 ELISA Kit, zgc:73274 ELISA Kit, wars ELISA Kit, MGC53671 ELISA Kit, WARS ELISA Kit, TrpRS ELISA Kit, WRS ELISA Kit, 85D-WRS ELISA Kit, CG9735 ELISA Kit, Dmel\\CG9735 ELISA Kit, WRS-85D ELISA Kit, l(3)03559 ELISA Kit, l(3)03560 ELISA Kit, l(3)3559 ELISA Kit, gamma-2 ELISA Kit, ifi53 ELISA Kit, ifp53 ELISA Kit, GB14934 ELISA Kit, ECK3371 ELISA Kit, JW3347 ELISA Kit, tryptophanyl-tRNA synthetase ELISA Kit, tryptophanyl-tRNA synthetase S homeolog ELISA Kit, Tryptophanyl-tRNA synthetase ELISA Kit, tryptophanyl-tRNA synthetase L homeolog ELISA Kit, tryptophan--tRNA ligase, cytoplasmic ELISA Kit, WARS ELISA Kit, wars ELISA Kit, wars.S ELISA Kit, Wars ELISA Kit, TrpRS ELISA Kit, wars.L ELISA Kit, LOC727582 ELISA Kit, trpS ELISA Kit, trpS2 ELISA Kit

Background:

Gene Name: Tryptophanyl tRNA Synthetase

Gene Aliases: GAMMA-2, IFI53, IFP53, WRS, TrpRS, hWRS, Tryptophan tRNA Ligase 1,Cytoplasmic, Interferon-induced protein 53, Tryptophanyl-tRNA synthetase

Gene ID: 22375

UniProt: P32921